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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Genetic Relationship among Bananas in AA, AAB and BB Groups Using Random Amplified Polymorphic DNA (RAPD) and Sequence Related Amplified Polymorphism (SRAP) Techniques) ผู้เขียน: สุจิตรา โพธิ์ปาน,  นางเบญจมาศ ศิลาย้อย, ศาสตราจารย์ , กวิศร์ วานิชกุล, ดร.สมศักดิ์ อภิสิทธิวาณิช, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractRAPD and SRAP techniques were introduced to analyse the genetic relationship among 29 accessions of banana in AA, AAB and BB groups at Department of Genetics and Department of Horticulture, Kasetsart University, Thailand. The genetic similarity and UPGMA were determined. The results showed that the SRAP technique was very similar to the RAPD technique for detecting genetic polymorphism and genetic relationship. The phylogenetic tree of RAPD and SRAP data showed two main clusters, AA-AAB and BB banana genome accessions. The BB group consisted of two subgroups with 8 cultivars. Within this group, Kluai Pa from Nakhon Si Thammarat and Kluai Pa from Na Khom, Kluai Tani Eisan and Kluai Tani Dam showed very close relationships. In the AA-AAB, it consisted of 21 cultivars. It was divided into three subgroups. First subgroup consisted of 6 from 10 AAB bananas. These cultivars were placed close to Kluai Pa Phrae. The second subgroup consisted of 4 AAB bananas, Kluai Klai, Kluai Nga Chang, Kluai Khom and Kluai Nom Sao, and all AA bananas except only ‘Kluai Pa Abisiania’ which was in the forth subgroup. The cultivar Kluai Klai and Kluai Nga Chang (AAB) were more closely related to Kluai Flava and Kluai Pa Pli Som while Kluai Khom and Kluai Nom Sao were closer to acuminata cultivars than the others. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Molecular Identification of Cycas by Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD)) ผู้เขียน:พัทธมน แสงอินทร์, นางอมรา ทองปาน, รองศาสตราจารย์, Anders J. Lindstrom, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractRAPD and RFLP were used to identify nineteen species of Cycas. Ten species of these Cycas namely C. chamaoensis, C. macrocarpa, C. pectinata, C. clivicola, C. pranburiensis, C. litoralis, C. tansachana, C. siamensis, C. nongnoochiae and C. simplicipinna are locally found in Thailand while the nine remaining species of C. seemannii, C. wadei, C. bougainvilleana, C. chevalieri, C. diannanensis, C. nathorstii, C. edentata, C. parvulus and C. micholitzii are from several countries around the world but collectively planted at Nong Nooch Tropical Botanical Garden. In the RAPD study, twenty random primers were screened to amplify the genomic DNA of nineteen species of Cycas. Only five primers, i.e., OPB-1, OPB-8, OPB-14, OPB-15 and OPB-17 of ten nucleotides long were found to give polymorphic DNA patterns. These eighty-seven bands of Cycas DNA at the size of 0.35 -2.5 kb could be used to indicate the differences of these Cycas. As for RFLP, three probes were synthesized from 5S rRNA gene, 5S rRNA repeat unit gene of C. clivicola and 18S rRNA gene of C. pranburiensis. The probes were hybridized with the genomic DNA of Cycas which had been digested with restriction enzymes BamHI, EcoRI and DraI. The phylogenetic trees were constructed based on their similarity index derived from DNA polymorphism of RAPD and RFLP separately. The RAPD data classified nineteen species of Cycas into two major groups which mostly corresponded to their geographic origins, i.e., one group of Thailand origin and another of other countries. However, the RFLP data gave a different set of grouping showing more to their morphological characteristics but less on their geographic origins. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Screening of Rubber Rootstock by the Assessment of Root Growth and Genetic Background) ผู้เขียน:Suneerat Wattanasilakorn, Sayan Sdoodee, Charassri Nualsri, Satthaya Bunratchoo สื่อสิ่งพิมพ์:pdf AbstractRubber seedlings from two early-introduced clones (EIRpsu 1 and EIRpsu 2) in southern Thailand were evaluated for root growth performance under field conditions. The two clones had been screened for white root disease tolerance. Genetic analysis of EIRpsu 1 and EIRpsu 2 was compared to RRIM 600 (the major cultivated variety of Hevea rubber in Thailand) using random amplified polymorphic DNA markers with 10 primers. Results from cluster analysis indicated that EIRpsu 2 was closer to RRIM 600 than EIRpsu 1 with similarity coefficients of 0.850, 0.860 and 0.890, respectively. Root growth of EIRpsu1 and EIRpsu2 was monitored at a rubber plantation in Langsuan district, Chumphon province during 2012–2013. Six-month-old seedlings of each clone were transplanted into a rhizobox. The experiment was designed as a randomized complete block design with four replications, with one plant per rhizobox. Shoot growth and root proliferation of the seedlings were recorded at two-monthly intervals from October 2012 to March 2013. The shoot growth was monitored by measuring plant height, trunk diameter and the number of compound leaves. It was found that RRIM 600 exhibited the highest shoot growth under field conditions, and it was significantly different from the other two clones. The root length density was measured by scanning the root systems of each seedling from a panel in the rhizobox. Results showed that the seedlings of EIRpsu 1 and EIRpsu 2 had better root growth than RRIM 600. The spatial distribution of roots indicated a rather deeper root system for the two selected clones. |
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 Researcherดร. พงศ์ระวี นิ่มน้อย, รองศาสตราจารย์ที่ทำงาน:ภาควิชาวิทยาศาสตร์และนวัตกรรมชีวภาพ คณะศิลปศาสตร์และวิทยาศาสตร์ สาขาที่สนใจ:ความหลากหลายทางพันธุกรรมจุลินทรีย์ Genetic diversity of microorganisms, Secondary Metabolites of microorganisms Resume |
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